The affinity purified rabbit anti-phospho-tyrosine antibody was conjugated to d-biotin via N-acetylation. The conjugates are useful for the detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. These conjugates have been utilized for non-radioactive tyrosine kinase assays.
250 μg/mL in PBS, 50% glycerol, 0.01% NaN3
The antibody was affinity purified with phospho-tyrosine immobilized on agarose.
The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.
ELISA (0.25 - 1.0 μg/mL); WB (0.25 - 1.0 μg/mL); IP (5 μg/mg protein); IHC (10 μg/mL)
Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine, which is considered a key step in the regulation of enzymatic activities such as signal transduction.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is one year from date of shipping if properly stored.
Product Specific References
1. J. Bio. Chem. 2006. 281: 37877-37887. doi:10.1074/jbc.M609398200