Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose.
Detection of a phosphorylated protein kinase with anti-phosphothreonine (ICP9807) Lane1-2: Untreated kinase Lane 3-4: Kinase treated with phosphatase PP1G
Western blot analysis of mouse extract spleen with ICP9807 A: Extract of spleen from mouse treated with sodium vanadate B: Extract of spleen from control mouse
250 μg/mL in PBS, pH 7, with 50% glycerol
Phospho-threonine KLH conjugates and phosvitin mixture
The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.
The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine.
Human and mouse
ELISA; WB; IP
Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
1. M.J. Betts, R.B. Russell. Bioinformatics for Geneticists, M.R. Barnes, I.C. Gray eds, Wiley, 2003.